Summary
Type: SPE
Application #: 300550
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
2 column volumes methanol, 2 column volumes dist. water
Sample application:
Slowly force or aspirate 5 ml urine or 2 ml serum or plasma through the column
Column washing:
2 ml water - acetone (8:2, v/v) or 12 – 13 ml water; dry column 3 min under vacuum
Elution:
After washing with water/acetone 2 x 500 μl methanol suffice, after washing
with plain water 12 – 13 ml methanol are required for steroid elution. The
methanolic solution can then be concentrated at 40 °C in a stream of nitrogen
Authors: Macherey-Nagel
Source: LC department